Plasmid

Part:BBa_K5322011:Design

Designed by: Xinyi Yang   Group: iGEM24_NJTech-China   (2024-09-28)


pET-29(a)-pT7-lac operator-(SOD-1+His)-T7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 577
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To ensure the successful expression of SOD-1 in Escherichia coli, we performed codon optimization of the SOD-1 sequence.


Source

The team obtains the SOD-1 sequence from BBa_K2215003.

References